Monoclonal Antibody Protocols EPUB
by William C. Davis
Part of the Methods in Molecular Biology series
EPUB
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Description
Since the initial description of techniques to immortalize anti- body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species.
Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines.
To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells.
The use of xenohybrids has minimized the problem of obtain- ing stable antibody-producing hybrids in all species examined thus far.
Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol- ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry.
The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono- clonal antibodies in biomedical, agricultural, and biological sciences.
The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga- tors, graduate and postgraduate fellows, and technical staff.
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- Format:EPUB
- Publisher:Humana Press
- Publication Date:03/02/2008
- Category:
- ISBN:9781592595327
Information
-
Download Now
- Format:EPUB
- Publisher:Humana Press
- Publication Date:03/02/2008
- Category:
- ISBN:9781592595327