Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitatsforschung : Volume 70 PDF

The processes involved in herpesvirus replication, latency, and oncogenic transformation, have, in general, been rather poorly defined.

A primary reason for this is the size and complexity of the herpesvirus genome.

Undoubtedly, a better understanding of the functions of the viral genome in infected and transformed cells will be achieved through studies with temperature-sensitive (ts) mutants of herpesviruses since, theoretically, any essential gene function can be affected by mutants of this type.

A. The Herpesviruses A consideration of the genetic analysis of members of the herpesvirus group necessitates a description, albeit brief, of the properties of the group and, most importantly, of their genetic material.

The herpesviruses comprise a group of relatively large (100-150 nm), enveloped viruses.

The envelope surrounds an icosahedral capsid enclosing a core which contains double- stranded DNA (ROIZMAN, 1969).

The group is thus defined on the basis of a common virion morphology.

In addition to a common structure, members of the group share a number of biological properties such as a similar replicative cycle, the ability to cause latent and chronic infections, and the ability to induce antigenic modifications of infected cell membranes.

Several herpes- viruses have been associated recently with malignancies in man and animals (KLEIN, 1972).

Herpesviruses are ubiquitous and have been described in over 30 different species (HUNT and MELENDEZ, 1969; WILDY, 1971; FARLEY et aI. , 1972; KAZAMA and SCHORNSTEIN, 1972; NAHMIAS et aI. , 1972; ROlZMAN et aI. , 1973). Their widespread occurrence in nature suggests a common ancestor.